AccuGO^TM Pfu DNA polymerase is a Pfu-based DNA polymerase specially provides both short extension time and high performance. It was designed to apply in the replication of DNA sequence required high fidelity and high efficiency. Like Pfu, AccuGO^TM Pfu also exhibits 3'→5' exonuclease activity that provides the proofreading ability and results in higher fidelity during DNA synthesis. Moreover, AccuGO^TM Pfu was engineered to have a better processivity than normal Pfu. Therefore, AccuGO^TM Pfu can easily amplify up to 10 kb blunt-ended DNA fragment from variant template. Even if the template’s GC content is up to 70%, AccuGO^TM Pfu still has good performance. In short, AccuGO^TM Pfu DNA polymerase is an ideal tool for molecular cloning containing long or complicated amplicons.